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. 2015 Aug 15;16:20. doi: 10.1186/s12860-015-0066-4

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© Morethson. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Mature primary osteoclasts were selected based on their morphology, using phase contrast optic system (Olympus IX70; 200 X) (a). In some experiments, it was performed further confirmation of the cell phenotype by immunofluorescence using antibody against calcitonin receptor (CTR) (confocal microscope Zeis LSM510; 200 X), a specific marker of osteoclasts (b), or by cytochemistry for tartrate resistant acid phosphatase (TRAP) (TRAP kit, Sigma, USA; 200 X) (c)