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. 2015 Aug 14;15:163. doi: 10.1186/s12866-015-0498-1

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© Bulir et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Chlamydia Outer Protein (Cop) B Interacts with T3S proteins. (a) GST-CopB_1–255 or GST-CopB_275–385 bound to glutathione-agarose beads (bait) pulled HisMBP-CdsF (prey) out of an E. coli lysate in the presence of a high salt wash buffer (500 mM NaCl). (b & c) Furthermore, GST-CopB fragments did not pull His-CopN or Cpn0803 out of an E. coli lysate in the presence of a high salt wash buffer