Fig. 5.
Inhibition of Chlamydia pneumoniae with CopB antibodies. Panels a-d show inhibition assay results performed with either no antibody (a), CopB antibody (b), pre-immune sera (c), or control antibody (α-GST) (d). Panel e shows the degree of inhibition by of CopB antibodies compared to control antibodies. Chlamydial inclusions are stained green, while HeLa cells are stained red by Evan’s blue counterstain. Panel f demonstrates reactivity of anti-CopB with (1) C. pneumoniae infected HeLa cell lysate, (2) uninfected HeLa cell lysate, (3) recombinant GST-CopB1–255 produced in E. coli, and (4) recombinant GST produced in E. coli. Experiments were performed in triplicate. Error bars represent 2 standard deviations. Images represent random fields of view. * = P < 0.0001