Table 12.17.3.
Tyrode's Solutions and Recommended Rhod-2 Injections for Different Species
| Component | Mouse | Rabbit | Guinea pig |
|---|---|---|---|
| NaCl | 112 mM | 130 mM | 130 mM |
| KCl | 5 mM | 4 mM | 4.75 mM |
| NaHCO3 | 25 mM | 24 mM | 25 mM |
| KH2PO4 | 1 mM | — | — |
| NaH2PO4 | — | 1.2 mM | — |
| MgSO4 | 1.2 mM | — | 1.2 mM |
| MgCl2 | — | 1.0 mM | — |
| CaCl2a | 1.8 mM | 0.75 mM | 1.25 mM |
| Glucose | 50 mM/(10 mM)b | 50 mM/(10 mM)b | 20 mM |
| Mannitolc | —/(45 mM)b | —/(45 mM)b | 45 mM |
| Rhod-2d | 20-40 μl | 0.4-0.6 ml | 0.1-0.2 ml |
a
To prevent calcium precipitation, gas the solution with CO2 prior to dissolving CaCl2.
b
Use the concentration in parentheses when preparing osmotic buffer.
c
Used as osmolarity buffer to keep the solution isotonic: can be substituted with dextran. This concentration can be changed to manipulate osmolarity by substituting glucose (Choi et al., 2006a,b).
d
Dye concentration at 1 mM: i.e., 1 mg/ml for rhod-2. Injection should be via bolus injection into the bubble trap, trickling directly into the cannula.