Fig. 5.
A: sample images of confocal microscopy comparing fluorescence intensity of dihydroxyethidium (DHE) (red color) in response to 80 and 180 mmHg of intravascular pressure in the endothelial and smooth muscle layers of mesenteric arteries of 6-mo-old rats. a and b are the endothelial layers; c and d are smooth muscle layers; a and c, and b and d are vessels treated with 80 and 180 mmHg of pressure, respectively. B: summarized histogram data detecting fluorescence intensity of DHE in the endothelial and smooth muscle layers of 6- and 24-mo-old rats. *Significant difference compared with 80 mmHg. #Significant difference compared with 6-mo-old rats. C: percent increase in fluorescence intensity from 80 to 180 mmHg of intravascular pressure. *Significant difference compared with 6-mo-old rats. #Significant difference compared with EC.