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. Author manuscript; available in PMC: 2015 Aug 14.
Published in final edited form as: J Am Chem Soc. 2013 Dec 12;135(51):19064–19067. doi: 10.1021/ja409896y

Figure 2.

Figure 2

UV–vis spectra of the WT, S90A, S90C, and F86Y mutant SHUV pigments. The F86Y mutant exhibits two distinct peaks. In the case of F86Y mutant, the relative population of USB and PSB is deeply dependent on the pH as indicated. We used IgorPro’s multipeak fitting function to deconvolute the F86Y spectrum into two Gaussian peaks with λmax (red lines) centered in the UV (376 ± 2 nm) and visible (432 ± 3 nm) regions.