Figure 4. Flowchart for the strategy to dissect detectable biomarkers from peripheral blood of the patients with STEMI.

We extracted 229 differentially overexpressed outlier genes in STEMI group compared with those of normal group by joining of two sets of experiments, and identified the top 10 of the screened genes encoding transmembrane/secretory molecules and highly expressed in the blood transcriptome using SignalP 3.0 to conduct ELISA for clinical validation.