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. 2015 Mar 29;6(15):13149–13163. doi: 10.18632/oncotarget.3693

Figure 1. Elevated levels of miR-497 or miR-34a inhibit cell proliferation.

Figure 1

(a) The relative levels of miR-497 and miR-34a were determined with the TaqMan® MicroRNA Assay and are expressed as fold change after normalization to the internal control, U6BsnRNA. Mean ± SEM, n = 3 (*P < 0.05, #P < 0.01, all vs. 16HBE cells). (b) A549, H460, and H1299 cells were transfected as described in Methods, and cell growth was monitored at 48 h using the CCK-8 assay. Mean ± SD, n = 3 (#P < 0.01 vs. NC mimic). (c) A549, H460, and H1299 cells were transfected, harvested at 48 h, and stained for cell cycle analysis on a FACSCalibur flow cytometer. Mean ± SD, n = 3 (*P < 0.05, #P < 0.01, all vs. NC mimic). (d) Representative histograms show the elevated levels of miR-497 and miR-34a that induced G0/G1 cell-cycle arrest in A549 cells; the percentages of cells in G0/G1 phase and S phase are shown.