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. 2015 Mar 29;6(15):13149–13163. doi: 10.18632/oncotarget.3693

Figure 6. miR-497 and miR-34a act synergistically by cotargeting CCNE1.

Figure 6

(a) Cyclin E1 protein levels in individually transfected A549 cells. Cyclin E1 protein levels were determined by immunoblotting using GAPDH as the loading control. A representative result of three independent experiments is shown. (b) Firefly luciferase reporter activity assay. A549 cells were transfected with Hi-miR497, Hi-miR34a, Hi-miR497/34a, or inhibitors. The relative luciferase activity was normalized to the Renilla luciferase activity and compared with that of the untransfected control. Mean ± SD, n = 3 (#P < 0.01 Hi-miR497/34a vs. Hi-miR497 or Hi-miR34a). (c) Hi-CCNE1a cells were transfected with empty vector or Hi-miR497/34a. Colony formation was examined in soft agar. (d) A representative result of the colony formation assay is shown (original magnification, ×40).