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. 2015 Apr 18;6(15):13164–13175. doi: 10.18632/oncotarget.3754

Figure 4. ARHGAP5 was a direct transcriptional target of miR-744 in NPC cells.

Figure 4

a. QRT-PCR and western blot analysis of ARHGAP5 expression in 5–8F and HONE1 cells transfected with miR-744 mimic, inhibitor, or controls for 48 h. b. MiR-744 putative binding sites with minimal minimum free energy (MFE) (Site 1, −508 to −484 and Site 2, −200 to −176) and their location are shown as nucleotide numbers relative to transcriptional start site (+1). c. Three different ARHGAP5 promoter constructs were generated: ARHGAP5 A and ARHGAP5 B containing both Site 1 and Site 2, ARHGAP5 C only containing Site 2. Luciferase activity of ARHGAP5 promoter-reporter constructs and vector control in the presence of miR-744 mimic or mimic-NC was analyzed in 5–8F and HONE1 cells. *p < 0.05; **p < 0.01; ***p < 0.001 compared to controls. N.S., non-significant.