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. 2015 Apr 23;6(15):13201–13215. doi: 10.18632/oncotarget.3915

Figure 4. miR-1 targeted cell cycle regulators CDK4, CDK6, CAPRIN1 and metastasis related gene Slug.

Figure 4

A. a. Schematic miR-1 putative target sites in 3′ UTRs of CDK4, CDK6, Caprin1 and Slug. b. Sequence of miR-1-mut. B. WB analysis of the protein levels of CDK4, CDK6, Caprin1, slug, Rb and p-Rb in response to 100 nM of indicated small RNA molecules. C. Luciferase assay. Luciferase reporters harboring putative target sites in the 3′ UTRs of Caprin1 (Caprin1-1 and Caprin1-2), CDK4, CDK6 and Slug, were co-transfected with 100 and 200 nM of indicated small RNA molecules in ACHN and 786-O cells. Relative luciferase activity was plotted as the mean ± SEM of three independent experiments. *, P < 0.05.