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. 2015 Mar 26;6(15):13255–13268. doi: 10.18632/oncotarget.3658

Figure 3. Ovarian spheroids enriched with CSCs highly express Btk signaling.

Figure 3

(A) The protein levels of Btk, Etk, Syk, p-PLCγ2, p-STAT3, and N-cadherin in parental (p) and spheroids (Sp) of ES-2 and Hey-A8 cells were assessed using western blotting. (B) In situ expression of Btk signaling was elucidated using 3D immunofluorescent staining. Antibodies against Btk, p-Btk, p-STAT3, and Sox-2 were used. Btk signaling was highly expressed and activated in ovarian spheroids, indicating the importance of Btk signaling in ovarian cancer stemness regulation.