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. 2015 Mar 26;6(15):13255–13268. doi: 10.18632/oncotarget.3658

Figure 4. Overexpression of Btk promotes STAT3 pathway activation and causes cisplatin resistance.

Figure 4

For the gain-of-function study, a Btk-overexpression clone was generated from OV-2008, a benign cell line that exhibits low Btk expression. (A) The expression of Btk and its downstream proteins was compared with that of control non-Btk-overexpressing cells by using immunoblotting. The overexpression of Btk promoted STAT3 pathway activation. Consequently, downstream effectors, such as Sox-2 and Bcl-XL, were upregulated. (B) After Btk was overexpressed, cells could easily form spheroids that represented the enriched CSC population.