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. 2015 Apr 19;6(15):13334–13346. doi: 10.18632/oncotarget.3871

Figure 4. RAL pathway-restricted gene expression in colorectal cancer cell lines.

Figure 4

A. Euclidean distance clustering of 20 top genes (having the lowest p value in the class comparison) responding to one of the three pathways (total number = 60). Clustering indicates that the similarity of the pathway-responsive target gene pattern exceeds the differences between cell lines. B. Venn diagram showing the overall number of genes up-regulated and down-regulated due to inhibition of the MAPK, PI3K and RAL pathway, respectively, in three colorectal cancer cell lines. The MAPK pathway was inhibited by treating cells with the MEK inhibitor U0126, the PI3K pathway with LY294002 and the RAL pathway with a specific siRNA. Quality control of microarray data is summarized in Supplemental Table 1. The complete normalized dataset including all cell line arrays is available in Supplemental Table 2. The list of 613 genes regulated by the RAL-pathway is depicted in Supplemental Table 3. The list of 554 genes regulated by the RAL pathway only is listed in Supplemental Table 4. The raw data is accessible in GEO by the accession number GSE39857.