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. 2015 Apr 15;6(15):13416–13433. doi: 10.18632/oncotarget.3826

Figure 7. Association of the stable accumulation of lipid droplets in TAMs with the deregulation of the PPARβ/δ target gene PDK4.

Figure 7

A. Staining of primary TAMs with Nile Red 0 h (ex vivo) and 4 d after plating in serum-free XV0 or R0 medium. B. Quantification of Nile Red stained TAMs (n = 3) treated as in A. C. L165,041 induction of PDK4 in MDMs (n = 3) and in TAMs (n = 3) cultured for 4d in ascites or R10 medium. D. Staining of MDMs with Nile Red before (d0) and after a 24-hour exposure to LA (d1), followed by a 4d fatty acid withdrawal in serum-free R0 medium (d1+4). E. Quantification of Nile Red stained MDMs (n = 2) before and after LA exposure as in D. F. L165,041 induction of PPARβ/δ target genes in MDMs (n = 4) pretreated with LA for 1 d, followed by a 4d serum-free R0 medium lacking fatty acids.