Figure 2. Myeloid cells from aged CpG-ODN+IFA-treated mice suppress T cell proliferation.

Spleens were collected from young and aged mice after ten days of CpG-ODN+IFA-treatment. (A) Splenocytes were stimulated with ConA or RPMI (unstimulated) and cultured for 72 h. (B) Naïve T cells isolated from young mice were stimulated with anti-CD3 plus anti-CD28 and co-cultured, at a 1:1 ratio, with myeloid cells purified from young or aged S.S or CpG-ODN+IFA-treated mice for 48 h. (A–B) T cell proliferation was measured by [³H]-Thymidine incorporation after 18 h pulse. Values are represented as c.p.m of stimulated minus unstimulated. (A–B) Data are representative of three independent experiments; mean ± SEM (n = 4 mice/group) **p < 0.01; ***p < 0.001.