Figure 10. Apoptotic inducement in Huh7 cells by GD55.

A. Annexin V binding assay. The Huh7 cells were infected with PBS, ZD55 or GD55 at an MOI of 5. 48 h later, the cells were stained with annexin V-FITC immediately followed by flow cytometry for apoptosis assay. Each value represents the mean of three wells. * < 0.01. B. Morphological observation of tumor tissue for cell apoptosis by TEM analysis. The obvious apoptosis was detected in tumors treated with GD55, secondly ZD55 than PBS treatments, such as nuclear collapse, appearance of nucleus deformation, and chromatin condensed in lumps et al.