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. 2015 Apr 12;6(15):13703–13717. doi: 10.18632/oncotarget.3800

Figure 1. Metabolic features and effects of resveratrol on glycolysis in HCC cell lines.

Figure 1

(A and B) Normalized lactate production and 2-DG uptake in HCC cells (SMMC-7721, Bel-7402, HepG2, HCC-LM3, Huh-7), and normal hepatic cells (QSG-7701 and LO2) within 72 h of culture under normoxic conditions. The lactate production was normalized to the level of QSG-7701 cell line. (C-E) Western blot analysis of HK2 and OXPHOS in total cell extracts from HCC cells and normal liver cells. β-actin was used as a loading control. The histogram represents the results of HK2 staining in three independent experiments (means ± s.e.m.). (F) O2 consumption in the indicated cell lines (nmol O2/million cells/min) was tested by a Clark-type oxygen electrode, which detected the concentration of dissolved oxygen in a closed chamber over time. (G and H) Relative lactate production and 2-DG uptake from HCC cell lines (HCC-LM3, Bel-7402, and Huh-7) in the absence or presence of resveratrol (20, 40, and 80 μM) within 72 h of culture under normoxic conditions. 2-DG uptake, lactate production, and O2 consumption were performed in triplicate and data represent the mean ± s.e.m. (*P < 0.05; **P < 0.01).