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. 2015 Apr 24;6(15):13757–13771. doi: 10.18632/oncotarget.3765

Figure 1. Interdependency of HDAC1 and HDAC2 expression in lung cancer cell lines.

Figure 1

A. HDAC1 and HDAC2 transcript expression in lung cancer cells. RNA extracted from a panel of normal lung and lung cancer cell lines was assessed for HDAC transcript expression by qRT-PCR. Cq values for test genes were normalized to actin and expressed as 2−[ΔΔCq] relative to the BEAS2B cell line. B. Co-extracted proteins from the same cell lines were immunoblotted for HDAC1 and HDAC2. For correlation in subsequent panels, HDAC protein levels were quantified using Odyssey analysis software, normalized to tubulin and shown relative to the BEAS2B cell line. C. HDAC1 and HDAC2 transcripts (top), but not proteins (below), exhibit strong positive correlation in the NSCLC and normal lung cell lines. D. HDAC1 mRNA (top), but not HDAC2 mRNA (below), is a good predictor of protein expression. Scatter plots represent quantification of protein (x-axis) plotted against that of mRNA (y-axis). E. Depletion of HDAC1 or HDAC2 leads to isoenzyme compensation. A549 cells were transfected with 40 nM siRNAs as indicated. Whole cell protein extracts were prepared 72 hr later and equivalent amounts immunoblotted. Expression of each HDAC was normalized to actin and is indicated relative to the siC control.