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. 2014 Oct 24;6(15):13822–13834. doi: 10.18632/oncotarget.2635

Figure 1. Features of EMT in the different steps of pancreatic carcinogenesis.

Figure 1

A, results of quantitative real-time PCR analysis of E-cadherin (CDH1) and vimentin (VIM) genes presented as the fold change in RNA expression between the gene of interest and β-actin. The mean values and SEM from 3 independent experiments conducted in quadruplicate are shown. ***, P < 0.001; *, P<0.05, by one-way ANOVA and Dunnett's multiple comparison post test. B, western blot analysis of EMT markers E-cadherin and vimentin. γ-tubulin was used as loading control. C, light-microscopic phenotype in HPDE, HPDE/KRAS, HPDE/KRAS/HER2/p16p14shRNA and HPNE, HPNE/KRAS, HPNE/KRAS/HER2/p16p14shRNA cell lines. D, levels of cell migration in HPDE and HPNE in vitro cell transformation model systems. Results are presented as percentages of the total distances between the wound edges enclosed by cells. The mean values and SEM from 3 independent experiments done in quadruplicate are shown. ***, P < 0.001 by one-way ANOVA and Dunnett's multiple comparison post test. Photographs of the wound area were taken by using phase-contrast microscopy immediately (0) and 24 hours after the incision.