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. 2014 Oct 24;6(15):13822–13834. doi: 10.18632/oncotarget.2635

Figure 2. Expression and secretion of ANGPTL2 and its receptor LILRB2 in the different steps of pancreatic carcinogenesis.

Figure 2

A, results of quantitative real-time PCR analysis of ANGPTL2 gene presented as the fold change in RNA expression between the gene of interest and β-actin. The mean values and SEM from 3 independent experiments conducted in quadruplicate are shown. ***, P < 0.001, by one-way ANOVA and Dunnett's multiple comparison post test. B, ELISA analysis of the secretion of ANGPTL2 in the conditional medium of HPDE and HPNE cell lines. Results are presented as percentages of the total protein extract from the respective secreting cells. The mean values and SEM from 3 independent experiments conducted in quadruplicate are shown. ***, P < 0.001; *, P < 0,05, by one-way ANOVA and Dunnett's multiple comparison post test. C, Western blot analysis for the expression of LILRB2 in HPDE and HPNE in vitro cell transformation model systems. Relative LILRB2 protein levels were quantified by the ratio between LILRB2 and γ-tubulin, which were arbitrarily set at 1.0 for control cells.