Skip to main content
. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Nutr Biochem. 2014 Jun 21;25(10):1066–1076. doi: 10.1016/j.jnutbio.2014.05.011

Figure 2.

Figure 2

A., B. Chromatin immunoprecipitation (ChIP) assay demonstrating MeCP2- and TR-glut4 interaction.

Top panel depicts representative 2% agarose gels demonstrating the input chromatin PCR amplified glut4 and gapdh control without an antibody (left panels), in the presence of nonspecific (IgG, -) and anti-polymerase II (pol II, +) IgGs (middle panels), and ChIP assay demonstrating the PCR amplified 384 bp fragment containing the glut4 DNA (top gels) or 230 bp fragment with the gapdh DNA (internal control) (bottom gels) from 100d old male MF and HC skeletal muscle in the presence of MeCP2 (A) or anti-TR (α1+β1) IgG (B) (respective right panels). Bottom panel depicts quantification (qPCR) of the 384 bp glut4 amplification product from the MeCP2 (A) or TR (α1+β1) (B) ChIP as a ratio to that of the respective gapdh DNA product after correction for the input control and expressed as a percent of MF. M = DNA size markers. Data is shown as mean±SEM, *p=0.016, n=4 (A) or *p=0.012, n=6 (B) samples in each group. TR (α1+2) ChIP demonstrated similar results as TR (α1+β1) (not shown).