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. Author manuscript; available in PMC: 2016 Aug 14.
Published in final edited form as: ACS Infect Dis. 2015 Jun 17;1(8):380–387. doi: 10.1021/acsinfecdis.5b00053

Figure 1.

Figure 1

The EBOV minigenome assay is robust in 96- and 384-well formats. (A) HEK293T cells were transfected with the components of the EBOV minigenome system, and 24 h post-transfection, cells were plated in a 96-well plate. “No VP35” indicates samples in which the plasmid expressing VP35 was replaced with pCAGGS empty vector. “VP35” indicates samples in which a complete polymerase complex, including VP35, was transfected. Forty-eight hours post-transfection luciferase activity was assessed. The data represent the mean and standard error of the mean (SEM) of 48 wells for each condition and are reported in relative light units (RLU). (B) HEK293T cells were transfected as in (A) and plated in a 384-well plate. At each time point post-transfection (hours post-transfection, hpt) luciferase activity was assessed. The data represent the mean and SEM of 16 wells each, reported in RLU; No VP35, black triangles; VP35, black circles. Z-Factor values were calculated in both (A) and (B) using the formula Z-factor = 1 − [(3σc+ + 3σc−)/(|μc+ − μc−|)].