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. Author manuscript; available in PMC: 2016 Aug 14.
Published in final edited form as: ACS Infect Dis. 2015 Jun 17;1(8):380–387. doi: 10.1021/acsinfecdis.5b00053

Figure 4.

Figure 4

Antiviral activity of hit compounds. To measure the antiviral activity of the compounds, Vero E6 cells were plated in a 96-well plate overnight and then pretreated with increasing concentrations of compound for 1 h, after which they were infected with EBOV-GFP at an MOI of 0.3. Three days post-infection the mean fluorescence intensity (MFI) was read to assess GFP expression and normalized to untreated controls to determine percent infection (left axis, black bars). In parallel, Vero E6 cells in a 96-well plate were treated with increasing concentrations of compounds, and ATP content was assessed 5 days post-treatment to determine cell viability (right-axis, gray bars). Data represent the mean and standard error of the mean (SEM) of triplicates.