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50 ng of recombinant Sph1, Sph2, Sph3, and Sph4 were subjected to SDS-polyacryamide gel electrophoresis in duplicate gels. One gel was stained with Coomassie blue (A), and the second was analyzed by Western blot with anti-Sph2 antiserum (B). Positions of molecular mass standards are shown in kilodaltons.
