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. 2015 Aug 14;9(8):e0003952. doi: 10.1371/journal.pntd.0003952

Fig 5. Hemolytic activity in spent growth medium of L. interrogans.

Fig 5

A. Hemolytic activity associated with spent growth medium from L. interrogans Manilae L495 and Pomona LC82-25 strains grown in EMJH, EMJH supplemented with 120 mM sodium chloride (Na) and EMJH supplemented with 120 mM sodium chloride and 10% rat serum (Na+serum) was examined on blood agar plates. 50 mU of Bacillus cereus sphingomyelinase (Bc SMase) was spotted onto the agar as a positive control. B. Hemolytic activities of spent growth medium from Manilae L495 and Pomona LC82-25 cultures incubated in EMJH and EMJH supplemented with 120 mM sodium chloride were measured by a quantitative hemolytic assay. The spent medium from the Pomona LC82-25 cultures were diluted 10 fold into EMJH plus 120 mM sodium chloride prior to conducting the assay. Measurements obtained with the diluted Pomona samples were multiplied by ten and plotted on the graph. Data are presented as mean ± standard deviation of triplicates. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (one-way ANOVA, Tukey multiple comparisons post-test).