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. 2015 Aug 14;9(8):e0003952. doi: 10.1371/journal.pntd.0003952

Fig 9. Hemolytic and sphingomyelinase activities secreted from L. interrogans sph2 mutant and complemented isolates.

Fig 9

Culture supernatant fluid from wild-type strain Lai L391, sph2 mutant, and sph2 mutant complemented with wild-type sph2 (TC4, TC8, and TC14) incubated in EMJH and EMJH with 120 mM sodium chloride for 24 hours was assayed for hemolytic activity (A) and sphingomyelinase activity (B). The dashed line indicates the background activity in culture medium (EMJH plus 120 mM NaCl) alone. Mean and standard deviation from three biological replicates are shown; *, p < 0.05; ***, p < 0.001 (one-way ANOVA, Tukey multiple comparisons post-test).