Table 1. List of oligonucleotides for PCR.
| Gene | Primer name* | Sequence (5’to 3’) # | Role |
|---|---|---|---|
| sph1 | lic12632(Nd)-3F | CTTACAcatatgAAACCTGGCAAACAAAATTCCATAAATC | P |
| lic12632(Xh)-4R | GCActcgagATGATGATAGATTAAATCTTTACTCCA | ||
| sph2 | lic12631(Kp)-17F | GACCATggtaccAGCGAGACGCTGAGTCTGA | P |
| lic12631(Xh)-18R | GTCCATctcgagGGTATTTTATTGAATAAGATTAGGAAAGT | ||
| sph3 | lic13198(Nd)-3F | AATTGTcatatgAAAGATTCATTAGTATACAATAAATTTTTA | P |
| lic13198(Xh)-4R | GCActcgagACGATAAATTAGATCCTTGCTCCAATCT | ||
| sph4 | lic11040(Nd)-3F | CTATCTcatatgGATTGTTTACCTGATTCACAGTCTTCTG | P |
| lic11040(Xh)-4R | CCTctcgagTTCCTCAGGGCCTTCATTCAATTGAACA | ||
| sph1 | lic12632-7F | TGAAACCGTTTTGATTACGGGTGA | Q |
| lic12632-8R | CGGCAAGTGCGTTTGTTTTTGTAT | ||
| sph2 | lic12631-11F | ATGTTGCCCACAAATCTTCCACG | Q |
| lic12631-12R | CCACATCTGTTTGATACGGATATTCTTCT | ||
| lipL41 | lipL41-7F | TCGGAAATCTGATTGGAGCGGAAGCA | Q |
| lipL41-8R | AGAAGCGGCGAAACCTGCCACT |
* F, forward primer; R, reverse primer
# Restriction sites in lower case
P, plasmid construction; Q, quantitative RT-PCR