Table 1. Summary of Illumina sequencing and assembly of the transcriptomes.

	Ruffe (G. cernua)	Flounder (P. flesus)
Sequences in pairs	87,169,850	78,943,030
Total nucleotides	7,407,977,682	6,695,503,487
Contigs	72,108	49,241
Contigs ≥ 500 bp	34,573	29,991
Max length (bp)	20,637	16,894
Min length (bp)	53	53
N50	1,828	2,106
Nucleotides	65,311,485	58,052,715

The Illumina reads were processed by trimming the adaptor sequences and removing low-quality sequences (cut-off q20). The sequences were assembled employing the CLC Genomics Workbench 7.5 (CLC Bio).