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This graph shows the effect of ACh (in the presence of 20 μM atropine) on the fluorescence response measured in CN21 cells. Data are displayed as the mean ± SEM of four replicates on a single plate. The sigmoidal concentration-response curve was fitted for concentrations between 3 x 10−7 and 3 x 10−4 M. Best fit values: EC50 = 5.0 × 10-6 M; Log EC50 = -5.3; Hill slope = 1.7
