Fig 9. Inhibition of hyaluronidase activity of ECV by AP and SLN.

Reaction mixture 300 μl contained 100 μg ECV in 100 mM acetate buffer pH 5.5, 150 mM NaCl and various concentrations of AP and SLN (0.1 nM-10 mM). The reactions were initiated by adding 50 μl substrate (hyaluronic acid) and incubated at 37°C for 2.5 h. After terminating the reaction, the contents were processed for color development.