Fig 4. Effects of antenatal DEX-treatment on receptor and transporter expression in total brains and brain endothelial fractions of PN4 pups.

Data are presented as the means ± SEM. For mRNA data, n = 5–6 biological samples; at PN4 2–3, brains from one litter were pooled to one biological sample. For western blotting, n = 6; at PN4, one brain represented one biological sample. Biological samples were collected from at least three different litters; claudin-5 data were normalized to the expression of the endogenous control GAPDH, samples from antenatally dexamethasone treated mice were compared to corresponding samples from NaCl treated mice, $: p<0.05 (two-tailed Student’s t-test).