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. 2015 Aug 14;10(8):e0136061. doi: 10.1371/journal.pone.0136061

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© 2015 Nadrah et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — A, Transwell migration assay of immature B cells from Socs3 ^fl/fl (SOCS3, red) or Socs3 ^+/+ (CTR, white) Mb1 ^Cre/+ mice towards CXCL12 in vitro. Bars indicate average and standard deviation. B, Distribution of GFP⁺ B lineage cells in BM of Socs3 ^fl/fl Mb1 ^Cre/+ mice (left) and Socs3 ^+/+ Mb1 ^Cre/+ (right) mice. Movement of GFP⁺ cells tracked for 30 minutes. Colored lines depict cell trajectories over time. Scale bar is 50 μm. C, Mean motility coefficient of B-lineage cells from Socs3 ^fl/fl Mb1 ^Cre/+ mice (red) and Socs3 ^+/+ Mb1 ^Cre/+ (black) mice. Cell displacement from starting coordinates is plotted against the square root of time. D, Measurement of cell axis ratio of GFP+ B lineage cells from Socs3 ^fl/fl Mb1 ^Cre/+ mice (red) and Socs3 ^+/+ Mb1 ^Cre/+ (black) mice. Lines indicate mean, circles depict individual cells analyzed.