Table 3. KD values of IgGs measured using KinExA 1 .
| Antibody | KD (×10-9M) | KD BoNT holotoxin | ||
|---|---|---|---|---|
| BoNT/A1 LC | A1 | A2 | A3 | |
| 7C8 | 1.39 (1.62–1.18) | 9.22×10−3 (11.00–7.62 × 10−3) | 2.89 × 10−3 (4.99–0.99 × 10−3) | 10.21 × 10−3 (13.84–7.09 × 10−3) |
| 10B12 | 44.59 (<87.71) | NB | ND | ND |
| 10F9 | 9.81 (<24.92) | NB | ND | ND |
| 11D8 | 20.85 (30.11–13.99) | NB | ND | ND |
| 12A11 | >1000 | 10.21 × 10−3 (12.49–8.17 × 10−3) | 7.34 × 10−3 (8.46–6.29×10−3) | 4.20 × 10−3 (<12.45 × 10−3) |
| 1D2 | 18.72 (26.39–11.79) | NB | NB | NB |
| 10B4 | 9.84 (13.21–6.49) | 0.51 (0.62–0.39) | ND | ND |
| ING2 | 115.43 (134.99–93.62) | 17.07 × 10−3 (19.23–15.08 × 10−3) | 14.24 × 10−3 (17.70–11.27×10−3) | NB |
| 5A20.4 | 3.12 × 10−3 (4.19–2.24 × 10−3) | 11.48 × 10−3 (18.01–6.59 ×10−3) | NB | NB |
1. Determined in a single measurement, 95% confidence intervals are shown in parentheses. ND: not determined, NB: no binding detectable.