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. 2015 Aug 14;10(8):e0133052. doi: 10.1371/journal.pone.0133052

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© 2015 O’Connell et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Transmitted light image of an atrial myocyte (top), Cav1.2 protein staining (green), α-actinin staining (red) and a merged image of Cav1.2, α-actinin and DAPI staining (bottom) under control (CTL; Panel A) and following incubation in stearic acid (SA; Panel B). Inset: 20 μm section of the merged image and corresponding intensity profile of Cav1.2 and α-actinin. Scale bars 20 μm. Panel C: Quantification of the intensity profiles shows SA did not alter the mean distance between intensity peaks for Cav1.2 (left) or α-actinin (right; n = 12, 12). Panel D: (left) Western blot for Cav1.2, with GAPDH as a control. Pane D: (right) normalized densitometry plot of Cav1.2 protein levels in CTL and SA treated cell lysates (n = 3).