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. 2015 Aug 14;9(8):e0003933. doi: 10.1371/journal.pntd.0003933

Fig 5. TA11145D AP2 domain (TaAP2.me1) fusion protein and PNE factor(s) bind to an (A)CACAC(A) type motif upstream of the TA11145 gene.

Fig 5

A. EMSA performed with 0.7μg of purified GST-TA11145D and 20 fmol of biotin-labelled double stranded oligo probe containing a double (A)CACAC(A) motif: lane 1, probe alone; lane 2, probe + GST-TA11145D; lane 3, mutant probe with both motifs mutated * denotes position of specific shift. B. EMSA performed with PNE and the double (A)CACAC(A) motif probe: lane 1, probe alone; lane 2, probe + PNE Day 0; lane 3, probe + PNE Day 9. Letters denote shifts detected, shift B was only present in Day 9 PNE. C. EMSA performed with PNE, and above probe: lane 1, probe only; lane 2, probe + PNE Day 9; lane 3, mutated probe + PNE Day 9, the infection associated shift detected in Day 9 PNE was not obtained with the mutant probe.