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. 2015 Aug 14;10(8):e0135688. doi: 10.1371/journal.pone.0135688

Table 1. Fungicide and adjuvant spray treatments were applied weekly on planted fields of Phacelia tanacetifolia and Sinapis alba in screened field cages where 20 uniquely paint-marked Osmia lignaria females were nesting.

Treatment sprays occurred on the evening of days 7 (April 13) and 14 (April 20) when bees were not active. The cage experiment was conducted at The Wonderful Company in Lost Hills, California in 2011.

Treatment Week 1, Week 2, Week 3 Application regime (date of spray) Application rate (kg/ha)
Cage Week 1 (Days 0–7) Week 2 (Days 8–14) Week 3 (Days 15–21) Week 2 Week 3
NS*, Water**, Water 1 NS Water Water
NS, N-90, N-90 2 NS N-90 N-90 0.4 0.4
NS, Rovral 4F, Rovral 4F 3 NS Rovral 4F Rovral 4F 2.2 2.2
NS, Pristine, Pristine 4 NS Pristine Pristine 1.6 1.6
NS, Rovral 4F, Pristine 5 NS Rovral 4F Pristine 2.2 1.6
NS, Pristine, Rovral 4F 6 NS Pristine Rovral 4F 1.6 2.2

* NS = no spray; nest initiation period to document normal nesting behavior

** Water was sprayed at equal volumes to treatment sprays