Fig 5. Level of NZB mtDNA across generations in a heteroplasmic mouse lineage produced by cytoplasmic transfer.

Circles depict the level of NZB mtDNA in mice estimated by ARMS-qPCR from ear biopsies. Filled circles depict females that were backcrossed (BC) with B6 males to obtain the next generation progeny (BC₁, BC₂, BC₃, BC₄ and BC₅). The level of NZB mtDNA was not determined for males, except in the founder lineage where males are depicted by empty circles. In the remaining generations (BC₁, BC₂, BC₃, BC₄ and BC₅) empty circles depict females that were not mated. Bars represent the means.