Table 1. Primer sequences used for measuring heteroplasmy and mtDNA copy number.
| Target | Primer | Sequence (5’-3’) a | Product (bp) |
|---|---|---|---|
| mtDNA b | MT12 | CGCCCTAACAACTATTATCTTCC | 736 |
| MT13 | GACCGTTTGTTTGTTGTTGAAA | ||
| mtDNA b | MT14 | CTCCGTGCTACCTAAACACCTTATC | 148 |
| MT15 | GACCTAAGAAGATTGTGAAGTAGATGATG | ||
| B6 mtDNA c | MT14 | CTCCGTGCTACCTAAACACCTTATC | 146 |
| ARMS2 | CCTAAGAAGATTGTGAAGTAGATGATGtC | ||
| NZB mtDNA d | MT20 | TGGCACTCCCGCTGTAAAAA | 118 |
| ARMS22 | TTATCCACGCTTCCGTTACGtC | ||
| Apob (nuclear DNA) | OIMR1544 | CACGTGGGCTCCAGCATT | 74 |
| OIMR3580 | TCACCAGTCATTTCTGCCTTTG |
aUnderlined nucleotides are complementary to one of mtDNA haplotypes (NZB or B6) due to the presence of a polymorphic nucleotide at position 3,599 (ARMS22) and 3,932 (ARMS2), respectively. A mismatched nucleotide introduced immediately 5’ to the polymorphic sites is represented in lowercase.
bNon-driscriminative assays that amplify mtDNA of B6 and NZB origin.
cDriscriminative assay that amplifies mtDNA of B6 origin.
dDriscriminative assay that amplifies mtDNA of NZB origin.