Skip to main content
. 2015 Aug 14;10(8):e0135533. doi: 10.1371/journal.pone.0135533

Fig 2. Vascular cells from the macrocirculation have specific SNA lectin binding.

Fig 2

All cell types were grown to confluence, media changed to serum free media for 1 hour, trypsinized, and stained for our lectin panel in Table 1 as described in methods. (A) MVECs show preferential binding for Griffonia simplicifolia (GS1) lectin as previously reported (95.43 ± 1.9%). (B) PASMCs have significantly more binding to Sambucus nigra (SNA1) and Maackia amurensis (MAA) than GS or Helix pomatia (HP) (100 and 79.43 ± 1.2% vs. 15.95 ± 3.0 and 15.58 ± 4.8%, respectively; P<0.05). (C) AOECs preferentially bind GS1 and SNA1 compared to HP and MAA (88.7 ± 5.7 and 100% vs. 23.48 ± 4.4 and 36.9 ± 1.8%, respectively. *P<0.05). (D) PASMC bind GS1 and SNA1 preferentially (97.9 ± 1.5 and 100% vs. 16.6 ± 2 and 18.93 ± 3.0%, respectively. P<0.05).