Fig 2. Dose-dependent suppression of systemic GFP silencing.

N. benthamiana 16C plants were analysed at 17 days after co-agroinfiltration of leaf L4 with pBinGFP and various RSS gene constructs. (A) Upper panel show the four top leaves of non-infiltrated 16C N. benthamiana. Vertical leaf positions are indicated in the lower panel (arrangement does not reflect angular leaf positions). (B) Four top leaves of 16C plants co-agroinfiltrated (at leaf L4) with GFP and GUS or P19 or TSWV NSs (in vector pBin19 and pK2GW7) with different OD₆₀₀ as indicated. (C) Visual Systemic Silencing Index (VSSI) of systemic GFP silencing in leaves L9 from plants as shown in panel B. (D) Digital Systemic Silencing Index (DSSI) of systemic GFP silencing in leaves L9 from plants as shown in panel B. Error bars indicate the standard error of the mean (SEM) of measures resulting from 10 leaves.