Extended Data Table 6. Ancient DNA libraries made from the Oase 1 mandible.
Metainformation | Sequencing results | All fragments | Deaminated fragments | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Library | Extract | UDG treatment | Index 1 | Index 2 | Extract used (μ1) | Sequences going into alignment | Sequences ≥35bp mapped | After dup. removal | Coverage | % C→T 5′end | % C→T 3′end | Coverage | % C→T 5′ end | % C→T 3′end |
A5227 | E1406 | Yes | ACTTGCG | AACTCCG | 8 | 206,982 | 118,976 | 34,486 | 112 | 8 | 19 | 5 | 19 | 36 |
A5252 | E1843 | Yes | GTAAGCC | TTGAAGT | 40 | 74,384 | 46,394 | 31,368 | 114 | 7 | 25 | 5 | 18 | 55 |
A9032 | E1406 | No | ATAACGT | ACTATCA | 6 | 9,321,903 | 5,904,210 | 51,810 | 178 | 20 | 21 | 12 | 31 | 39 |
A9033 | E1406 | No | AATAGGA | ACCAACT | 6 | 7,932,271 | 4,816,314 | 55,878 | 193 | 21 | 20 | 13 | 36 | 38 |
A9034 | E1406 | No | ATCACGA | AACTCCG | 6 | 10,422,467 | 6,861,634 | 59,883 | 207 | 20 | 20 | 14 | 35 | 38 |
| ||||||||||||||
All | Both | Mix | 27,958,007 | 17,747,528 | 233,425 | 803 | 17 | 21 | 49 | 30 | 39 |
Contamination estimate based on the direct comparison of C→T substitutions of all fragments and deaminated fragments only.