Figure 1.

N-6 binds to RXRα. (A) Structure of NSC-640358 (N-6). (B) RXRα-LBD protein was incubated with [³H]9-cis-RA in the presence or absence of unlabeled 9-cis-RA or N-6. Bound [³H]9-cis-RA was quantitated by liquid scintillation counting. (C) Gradient concentrations of N-6 were injected through flow cells immobilized with RXRα-LBD. The kinetic profiles are shown and the dissociation constant (K _d) of the N-6/RXRα-LBD complex was calculated to be 15.755 × 10⁻⁶ mol/L. (D) RXRα-LBD protein enhanced fluorescent intensity of N-6 (ex 278 nm, em 338 nm, cutoff 325 nm, delay 50 μs, integration 450 μs). One of three similar experiments is shown