Figure 3.
N-6 inhibits TR3 transcriptional activity by binding to RXRα. (A–D, and F) MCF-7 cells cotransfected with pG5-Gaussia-Dura reporter vector and the indicated expression vectors were treated with or without N-6 (10 μmol/L), UVI3003 (1 μmol/L), and 9-cis-RA (10−7 mol/L) for 18 h. Reporter activities were measured and normalized. Data shown are mean ± SD (*P < 0.05). (E) HEK293T cells cotransfected with pCMV-Myc-TR3 and pBIND-RXRα-LBD expression vectors were treated with UVI3003 (1 μmol/L) or N-6 (10 μmol/L) for 18 h. Cell lysate were analyzed for heterodimerization of Nur77 and Gal4-DBD-RXRα-LBD by co-immunoprecipitation with anti-myc antibody. One of three similar experiments is shown