FIG. 2.

Infrared laser thermocycler comprising two infrared laser sources controlled via a common power supply, two solenoid shutters, and a polymer microchip featuring two 1 μl reaction chambers. (a) Close-up photograph of one of the reaction chambers shows the ports, used for sample loading and pressurization, fill channels, 1 μl reaction chamber, and the tip of the embedded thermocouple bonded between the layers of the microchip. (b) Simplified schematic and (c) photograph show the basic components of the thermocycler assembly.