Table 1.

Sensitivity of FP-Aeq variants derived from best-fit curve of in vitro calibration data

	CLZ-native					CLZ-f			CLZ-hcp
	Aeq [21]	CitA	GA [4]	tdTA	Redquorin	CitA	tdTA	Redquorin	Redquorin
EC50 (μM)	1.27	0.56	0.64	1.13	1.53	0.26	0.61	0.79	0.31
Relative intensity at pCa 6	1.0	39.4	17.8	1.6	1.0	465.7	19.3	14.8	335.0
Log (L/Lmax) at pCa 6	−3.44	−1.85	−2.19	−3.24	−3.43	−0.78	−2.16	−2.27	−0.92