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. Author manuscript; available in PMC: 2015 Aug 15.
Published in final edited form as: Immunol Lett. 2014 Sep 26;162(2 0 0):264–272. doi: 10.1016/j.imlet.2014.09.016

Fig. 2.

Fig. 2

Gating strategy for CD4 T-cell proliferation using carboxy fluorescein succinimidyl ester (CFSE) dye. (A) Total lymphocyte gate, (B) live/dead lymphocytes, (C) the CD3+ CD4+ T-cell gate, (D) unlabeled CD3+ CD4+ T-cells, (E) unstimulated CD3+ CD4+ T-cells (CFSE negative control) and (F) CD4 T-cell proliferation (CD3+ CD4+ CFSE−). T-cells, with PHA stimulation as a positive control.