Figure 5.

JNK Activation Promotes the Clonal Expansion of Wild-Type Cells

(A and B) puc-LacZ expression (α-β-Gal staining) in 3-day-old WT (FRT82B, puc^A251/TM6B) (A) and M^−/+ (B) guts (FRT82B, puc^A251/FRT82B, RpS3^∗).

(C and D) Representative images of WT clones in M^−/+ guts with (D and D′) or without (C and C′) continued expression of Puc in all progenitor cells and ECs from the time of clone induction (−RU486 and +RU486, C and D, respectively; hsflp/+; PSwitch^ALL/UAS Puc; FRT82B, ubiGFP, RpS3/FRT82B).

(E) Analysis of clone-size distributions for WT clones either in WT guts (left) or in M^−/+ guts (middle and right graphs), with (dark blue) or without (light blue) continued expression of Puc (as in C and D). Genotypes: left: hsflp/+; PSwitch^ALL/UAS Puc; FRT82B, ubiGFP/FRT82B; middle: hsflp/+; PSwitch^ALL/UAS Puc; FRT82B, ubiGFP, RpS3/FRT82B; right: hsflp/+; PSwitch^PC/UAS Puc; FRT82B, ubiGFP, RpS3/FRT82B. p values (Mann-Whitney test) are indicated above each experiment.

Scale bars represent 50 μm. See also Figure S3.