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. 2015 Aug 10;34(3):297–309. doi: 10.1016/j.devcel.2015.06.010

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

JAK-STAT Activation Fuels the Clonal Expansion of Wild-Type Cells

(A and B) Expression of GFP driven by upd3-Gal4 in 5-day-old WT (A) (Upd3Gal4, UASGFP/+; FRT82, tub-CD2-DsRed/+) and M^−/+ (B) guts (Upd3Gal4, UASGFP/+; FRT82, tub-CD2-DsRed, RpS3/+).

(C and D) Representative images of WT clones either in M^−/+ guts (C and C′; hsflp/+;; FRT82B, tub-CD2-DsRed, RpS3/FRT82B) or in M^−/+ guts with reduced JAK-STAT activity by removal of one functional copy of dome (dome^−/+) (D and D′; hsflp/dome^G0218;; FRT82B, tub-CD2-DsRed, RpS3/FRT82B).

(E) Analysis of clone-size distributions for WT clones either in WT guts or in M^−/+ guts, with (dark blue) or without (light blue) reduction of JAK-STAT pathway activity by expression of Dome^DN (PS^All, ±RU486) or by removal of one functional copy of dome (dome^−/+). Genotypes: left: hsflp/+; PSwitch^ALL/UAS Dome^DN; FRT82B, ubiGFP, RpS3/FRT82B; middle: [WT/WT dome^+/+] hsflp/+;; FRT82B, tub-CD2-DsRed/FRT82B; [WT/WT dome^−/+] hsflp/dome^G0218;; FRT82B, tub-CD2-DsRed/FRT82B; right: [WT/M^−/+ dome^+/+] hsflp/+;; FRT82B, tub-CD2-DsRed, RpS3/FRT82B; [WT/M^−/+ dome^−/+] hsflp/dome^G0218;; FRT82B, tub-CD2-DsRed, RpS3/FRT82B. p values (Mann-Whitney test) are indicated above each experiment.

Scale bars represent 50 μm. See also Figure S4.