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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Shock. 2015 Sep;44(3):258–264. doi: 10.1097/SHK.0000000000000410

Figure 2.

Figure 2

Figure 2

Figure 2

(A) Representative Oxygraph traces for respirometry experiments showing mass specific respiratory fluxes following titration of substrates and then GDP. Group mean data from sham (n=10) 1 day post burn (n=8) and 10 days post burn (n=9) are plotted. (B) Total uncoupled respiration per mg of tissue after the addition of substrates increases after burn injury. (C) UCP1 independent respiration per mg of tissue following titration of GDP marginally increased after burn trauma. (D) GDP flux control ratio was reduced after burn injury, indicating greater GDP sensitivity and thus UCP1 function. (E) UCP1 dependent respiration per mg of tissue, i.e., the change in respiration after GDP titration significantly increased after burn trauma, demonstrating the induction of thermogenically competent mitochondria in iWAT following severe burns. DPB = days post burn. *P<0.05, **P<0.01 and ***P<0.001 vs. sham. +P<0.05, ++P<0.01 and +++P<0.001 vs. 1 DPB. ∞P<0.05 vs. 10 DPB.